| 產(chǎn)品名稱 | ME-180 |
|---|---|
| 商品貨號 | B165137 |
| Organism | Homo sapiens, human |
| Tissue | cervix; derived from metastatic site: omentum |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain human papilloma virus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | epidermoid carcinoma |
| Age | 66 years |
| Gender | female |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | hyperdiploid to hypohexaploid with abnormalities including dicentrics, fragmentation and markers; unstable; (P63) hypotriploid with abnormalities including dicentrics and acrocentric fragments, large submetacentric and large subtelocentric markers |
| Derivation | This line was derived from a highly invasive squamous cell carcinoma with irregular cell clusters and no significant keratinization. |
| Clinical Data | 66 years Caucasian female |
| Antigen Expression | Blood Type A; Rh+; HLA A1, A11, B5(+/-), B40 |
| Oncogene | p53 +; pRB + |
| Genes Expressed | Oncogenes: p53 +; pRB + |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I) |
| Comments | Desmosomal attachments were observed between cells in monolayer culture and cytoplasmic tonofilaments were noted.
Contamination with Mycoplasma arginini was detected and eliminated in 1970.
Growth of ME-180 cells is inhibited by tumor necrosis factor alpha (TNF alpha).
The cells contain human papillomavirus (HPV) DNA with greater homology to HPV-68 than HPV-18. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37.0°C |
| STR Profile | Amelogenin: X CSF1PO: 11 D13S317: 11,13 D16S539: 12,13 D5S818: 12 D7S820: 9,10 THO1: 8,9.3 TPOX: 8,10 vWA: 15,17 |
| Isoenzymes | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor | JA Sykes |
| Deposited As | Homo sapiens |
| U.S. Patent Number | 5,981,173 |
| References | Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Sykes JA, et al. Some properties of a new epithelial cell line of human origin. J. Natl. Cancer Inst. 45: 107-122, 1970. PubMed: 4317734 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Reuter S, et al. Characterization of a novel human papillomavirus DNA in the cervical carcinoma cell line ME180. J. Virol. 65: 5564-5568, 1991. PubMed: 1716694 Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 van Dijk MA, et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997. PubMed: 9270016 St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Longuet M, Beaudenon S, Orth G. Two novel genital human papillomavirus (HPV) types, HPV68 and HPV70, related to the potentially oncogenic HPV39. J Clin Microbiol 34(3): 738-744, 1996. PubMed: 8904450 Cellosaurus ME-180 (CVCL_1401). U.S. Patent 5,981,173 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
